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KMID : 0380620130450060770
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 6 p.770 ~ p.776
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Antioxidant and Anti-cancer Activities of Squash (Cucurbita moschata Duch.) Leaf Extract In vitro
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Kwak Youn-geun
Ju Ji-Hyeung
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Abstract
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The aim of this study was to investigate the antioxidant and anti-cancer activities of squash leaf extract (SLE) in vitro. The total polyphenol and flavonoid levels of SLE were 263.4 mg gallic acid equivalent/100 g and 73.6 mg quercetin equivalent/100 g, respectively. The radical-scavenging activity of SLE at the concentration of 300 μg/mL was 69.4%. SLE significantly inhibited human cancer cell growth (by 60.6-87.9% in HCT116 colon cancer cells and by 73.4- 86.4% in H1299 lung cancer cells at the concentrations of 37.5, 75, and 150 μg/mL) and attachment (by 28.4% in HCT116 and by 16.8% in H1299 at the concentration of 150 μg/mL). SLE also altered nucleus morphology and increased nuclear staining intensity (by 42.8-58.2% in HCT116 and by 25.5-32.9% in H1299 at the concentrations of 37.5 and 75 μg/mL), indicating its apoptosis-inducing activity. These results demonstrate the antioxidant and anti-cancer activities of SLE in vitro.
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KEYWORD
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squash leaf, antioxidant activity, cancer, cell growth, attachment
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